Hematoxylin and Eosin Stain Kit
The Hematoxylin and Eosin Stain Kit is intended for use in histology and cytology applications. Included in this kit is a newly formulated Eosin that provides the benefits of a traditional alcoholic formula with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, reduced tendency to spill over container, hands, and countertops, and improved surface tension to remain on tissue section. Our Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.
Cytoplasm: Light Pink
Collagen: Pink
Muscle: Pink/Rose
Erythrocytes: Pink/Red Nuclei: Blue
Summary Protocol:
Deparaffinize and hydrate.
Apply adequate Hematoxylin, Mayer's (Lillie's Modification) and incubate for 5 minutes.
Apply adequate Bluing Reagent and incubate for 10-15 seconds.
Apply adequate Eosin Y Solution (Modified Alcoholic and incubate for 2-3 minutes.
Clear and mount.
HAE-1 Kit Contents:
Hematoxylin, Mayer's (Lillie's Modification), 500ml, Item #: HMM500
Bluing Reagent, 500ml, Item #: BRT500
Eosin Y Solution (Modified Alcoholic), 500ml, Item #: EYB500
HAE-2 Kit Contents:
Hematoxylin, Mayer's (Lillie's Modification), 30ml, Item #: HMM030
Bluing Reagent, 30ml, Item #: BRT030
Eosin Y Solution (Modified Alcoholic), 30ml, Item #: EYB030