TTF-1 & Cytokeratin 5 Multiplex Cocktail; Clones 8G7G3/1 & EP42 (Ready-To-Use)
Species: Mouse & Rabbit
Designation: Mouse Monoclonal & Rabbit Monoclonal
Clones: 8G7G3/1 & EP42
Isotype: IgG1 & IgG
Species Reactivity: Human. Others not tested.
Positive Control: Adenocarcinoma of the Lung or Thyroid for TTF-1. Skin for normal tissue and Mesothelioma for abnormal tissue for Cytokeratin 5.
Principle: Multiplex IHC staining allows simultaneous testing of two or more analytes on a single tissue section utilizing different colors for each antigenic target.
Specificity: This antibody reacts with TTF-1 protein found in adenocarcinomas of the lung and tumors originating in the thyroid. TTF-1 positive cells are found in Type II pneumocytes and Clara cells in the lung. In the thyroid, follicular and parafollicular cells are positive. In lung cancers, Adenocarcinomas are usually positive, while Squamous Cell Carcinomas and Large Cell Carcinomas are rarely positive. In addition, Small-Cell Carcinomas (of any primary site) are usually positive.
Cytokeratin 5 is expressed in many non-keratinizing stratified squamous epithelia including: basal epithelia, hair follicles, trachea, tongue mucosa, as well as basal cells in prostate glands and myoepithelial cells in mammary glands. Cytokeratin 5 protein is also found in most epithelial and bispasic mesotheliomas, large cell carcinoma and pulmonary squamous cell carcinomas.
This Multiplex cocktail of TTF-1 and Cytokeratin 5 produces two-color high contrast staining for differentiating primary adenocarcinoma of the Lung from Metastatic Carcinomas of the breast and Malignant Mesothelioma.