CRF Anti-Polyvalent HRP Polymer (DAB) Stain Kit
The CRF Anti-Polyvalent HRP Polymer (DAB) Stain Kit based on proprietary CRF Technology has been developed to provide the cleanest, most consistent staining available. Developed in the research laboratories of ScyTek, the system utilizes a polymerized peroxidase label that eliminates biotin and its' associated background issues from the equation. In addition, this product reduces the steps required for immunohistochemical staining by combining two steps from the traditional Biotin-Streptavidin system. The CRF technology based Anti-Polyvalent system is effective with antibodies of mouse, rat, rabbit and guinea pig.
Summary Protocol:
Incubation with an unconjugated primary antibody specific to the target antigen.
The primary antibody is bound to the biotinylated secondary antibody.
The secondary antibody is then bound to the HRP-Labeled streptavidin.
A brown color is created when the HRP is bound to site of the primary antibody by reacting with DAB.
Kit Components:
Peroxide Block- 1 x 8ml - ACA008
Super Block - 1 x 8ml - AAA008
CRF Anti-Polyvalent HRP - 1 x 8ml - ABZ008
DAB Chromogen - 1 x 3ml - ACB003
DAB Substrate - 7 x 5ml - ACC005
Items recommended but not included:
Citrate Plus - 1 x 500ml - CPL500
Hematoxylin for Automation - 1 x 500ml - HAQ500
Bluing Reagent - 1 x 500ml - BRT500
Alternate CRF Polymer IHC detection kits:
Anti-Mouse HRP-DAB IHC kit - PolyTek Anti-Mouse (DAB) Polymerized HRP Imaging System - PIR080
Anti-Rabbit HRP-DAB IHC kit - PolyTek HRP Anti-Rabbit Polymerized Imaging System - PIP080