
The UltraTek line is our leading edge system, designed to provide optimal staining with incubation times of 10 minutes each for the link antibody and enzyme label. UltraTek Anti-Polyvalent is specially formulated and optimized to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique requires the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. For most procedures, commercially available primary antibodies can be diluted up to 50% more than with other systems.
Summary Protocol:
Incubate on tissue section after application of the primary antibody.
Calışkan, Eray et al. “The effect of copper intrauterine devices on the expression of mucin 1 and integrin β1 in the luteal phase endometrium.” Journal of the Turkish German Gynecological Association vol. 10,4 194-8. 1 Dec. 2009