The Gram Stain Kit (Modified Brown & Brenn) is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria.
Gram Positive Bacteria: Blue
Gram Negative Bacteria: Red
Background: Yellow
Nuclei: Red
Isenberg, H.H. Clinical Microbiology Procedures Handbook. American Society for Microbiology, 1992.
Sheehan, DC., Hrapchak, BB. Theory and Practice of Histotechnology; 1980, page 235.
Brown, J.H., Brenn, L. A method for the differential staining of gram-positive and gram-negative bacteria in tissue sections. Bulletin John Hopkins Hospital, 1931, Volume 48, pages 69-73.
Gram, C. Fosrtchr. Med., Volume 2, page 185, 1884.