Glycogen, Elastin, Mucin Stain Kit
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Product Information
This stain kit utilizes a sulfation procedure and Toluidine Blue O to give specific metachromatic staining of Glycogen, Elastin, and Mucin (acidic and neutral) in formalin-fixed paraffin-embedded sections. Similar methods have previously been studied and referenced as Aldehyde Bisulfite Toluidine (ABT) and Permanganate Bisulfite Toluidine (PBT) staining. Glycogen may be visualized with a contrasting background as an improvement upon PAS staining.
Literary References
1. Makovitzky J, Richter S. The relevance of the aldehyde bisulfite toluidine blue reaction and its variants in the submicroscopic carbohydrate research. Acta Histochem. 2009;111(4):273-91. doi: 10.1016/j.acthis.2008.11.027. Epub 2009 Jan 20. PMID: 19157525; PMCID: PMC7172417.
2. Richter S, Makovitzky J. Topo-optical visualization reactions of carbohydrate-containing amyloid deposits in the respiratory tract. Acta Histochem. 2006;108(3):181-91. doi: 10.1016/j.acthis.2006.01.002. Epub 2006 Mar 15. PMID: 16542712.
3. Malinin GI. Metachromatic staining of sodium bisulfite addition derivatives of glycogen. J Histochem Cytochem. 1970 Nov;18(11):834-41. doi: 0.1177/18.11.834. PMID: 4100992.
4. KRAMER H, WINDRUM GM. Sulphation techniques in histochemistry with special reference to metachromasia. J Histochem Cytochem. 1954 May;2(3):196-208. doi: 10.1177/2.3.196. PMID: 13163394.
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