
5-Bromo-4-Chloro-3-Indolyl Phosphate/nitroblue Tetrazolium (BCIP/NBT) Solution
Is a widely used chromogen for immunohistochemical and in-situ staining. This reagent is available as a single component, ready-to-use reagent. When in the presence of the Alkaline-Phosphatase enzyme, BCIP/NBT produces a purple precipitate that is soluble in alcohol.
1. Salamon, P, Shefler, I, Moshkovits, I, et al. IL-33 and IgE stimulate mast cell production of IL-2 and regulatory T cell expansion in allergic dermatitis. Clin Exp Allergy. 2017; 47: 1409– 1416. https://doi.org/10.1111/cea.13027
2. Yao, Z., Li, Y., Yin, X., Dong, Y., Xing, L. and Boyce, B.F. (2014), NF-κB RelB Negatively Regulates Osteoblast Differentiation and Bone Formation. J Bone Miner Res, 29: 866-877. https://doi.org/10.1002/jbmr.2108
3. Van-Yu Wu, Clyde W. Shearman, Margo P. Cohen, Identification of Calnexin as a Binding Protein for Amadori-Modified Glycated Albumin, Biochemical and Biophysical Research Communications, Volume 284, Issue 3, 2001, Pages 602-606, ISSN 0006-291X, https://doi.org/10.1006/bbrc.2001.4982